Determination of the optimum concentration of the coupling agent in chloramphenicol analysis

The aim of this research is to determine the optimum concentration coupling agent used in chloramphenicol analysis, then calculate limit detection that photometric reaction. Chloramphenicol a colorless compound often abused animals whose products are consumed by humans. has side effects on human body. So, must be analyzed. To facilitate analysis process, converted into colored compounds called azo compounds. make go through process reduction, diazotization, and coupling. A an important played role formation was analyzed using UV-Vis spectrophotometer. This research, N-(-1-Naphthyl) ethylenediamine dihydrochloride (NEDA) with variations concentrations 0.1%, 0.3%, 0.5% at 565 nm. gives maximum absorbance 0.1%. (LOD) obtained 0.0498 μg/mL, quantitation (LOQ) 0.1660 sensitivity value 0.1425, correlation coefficient (R2) 0.999 range 0,14 - 1,4 ppm.